Sampling techniques for single-cell electrophoresis

Sampling efficiency of a single-cell capillary electrophoresis system.

Capillary electrophoresis (CE) combined with a laser-induced fluorescence (LIF) detection scheme is a powerful approach for single-cell analysis. For measurements requiring a high temporal resolution, CE-LIF is often combined with cell lysis systems based on pulsed lasers. Although extremely rapid, laser lysis has raised some concerns about the efficiency at which the cell contents are sampled....

Nail Sampling techniques for Diagnosing of onychomycosis-Section 10

The clinical suspicion of onychomycosis based on appearance of the nails, requires fungal culture for confirmation. The unfamiliar terminologies used in reporting culture results leads to misinterpretation and hamper treatment decision. Accurate laboratory diagnosis of onychomycosis depends on the quality of sample collection, and reporting by microbiologist. Site selection for sampling depends...

Evaluation of DNA damage in Cyprinus carpio (L. 1758) exposedto malathion using Single Cell Gel Electrophoresis

Malathion (S-(1, 2-dicarboethoxyethyl) O, O-dimethyl phosphorodithioate) is one of the organophosphate pesticides comprehensively used in agriculture fields throughout the world. In spite of widely used, little information are available about DNA damage in aquatic organisms. Therefore, the present study carried out to investigation of DNA damage induced by malathion in Cyprinus carpio (Pisces: ...

Assessment of DNA damage induced by butachlor on Cyprinuscarpio(L. 1758) using Single Cell Gel Electrophoresis

Butachlor (N-(butoxymethyl)-2-chloro-N-2, 6-diethylacetanillide) is widely used chloroacetanilide herbicide in Asian region, especially in the north of Iran. Several studies have been reported on toxicity of butachlor, but little information is accessible on DNA damaging potential. Thus, the present study was undertaken to investigate of DNA damage by butachlor in Cyprinuscarpio(Pisces: Cyprini...

Single Cell Gell Electrophoresis (Comet Assay)